吴瑛,张勇,姚合斌.高脂联合小剂量链脲佐菌素建立实验性2型糖尿病大鼠模型[J].转化医学杂志,2017,6(6):355-357
高脂联合小剂量链脲佐菌素建立实验性2型糖尿病大鼠模型
Study of streptozotocin and high fat diet inducing type 2 diabetes in a rat model
  
DOI:
中文关键词:  2型糖尿病  血糖  高脂  链脲佐菌素  模型,大鼠
英文关键词:Type 2 diabetes mellitus  Blood glucose  High fat  Streptozotocin (STZ)  Model, rat
基金项目:解放军总后勤部保健专项课题(CWS14BJ38)
作者单位
吴瑛 海军总医院营养科 
张勇 中国人民解放军32150部队医院 
姚合斌 海军总医院内分泌科 
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中文摘要:
      目的探讨建立具有胰岛素抵抗、近似人2型糖尿病特征的SD大鼠模型。方法将36只SD大鼠随机分为A组(对照组即普通饲料组)6只、B组(高脂饲料组)6只、C组[普通饲料+小剂量链脲佐菌素(streptozotocin,STZ)组]12只、D组(高脂饲料+小剂量STZ组)12只共4组。C组、D组大鼠分别在普通饲料和高脂饲料喂养4周后使用小剂量STZ(30 mg/kg)腹腔注射5 d。随机血糖>16.7 mmol/L且持续2周为建模成功。结果C组、D组能建立2型糖尿病SD大鼠模型,成模率分别为66.7%(8/12)、91.7%(11/12)。结论高脂饲料+小剂量STZ发病过程接近自然,成模率更高,模型稳定,是值得推广的2型糖尿病动物模型。
英文摘要:
      ObjectiveTo set up a SD rat model similar to human type 2 diabetes mellitus with insulin resistance. MethodsThirtysix SD rats were randomized into four groups, normal group (group A, n=6), highfat diet group (group B, n=6), lowdoes streptozotocin group [group C, streptozotocin (STZ), 30 mg/kg, n=12], and highfat diet and lowdoes STZ (30 mg/kg) group (group D, n=12). Group C and D were induced by an injection of lowdoes STZ (30 mg/kg) after 4 weeks of normal chow feeding and highfat chow feeding respectively. Rats with random plasma glucose levels >16.7 mmol/L for 2 weeks were modeling success. ResultsBoth normal chow feeding associated with lowdoes STZ (30 mg/kg) and highfat chow feeding associated with lowdoes STZ (30 mg/kg) can establish type 2 diabetes animal model. The diabetes morbidity was 66.7% (8/12) in group C, and 91.7% (11/12) in group D. ConclusionGroup D has moderately high blood sugar, insulin resistance, the success rate is high, and can be used as a good model of type 2 diabetes mellitus in relevant research.
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