杨超,卢伟,马军朋,杨剑,蒋富强,杜鹏,张嘉诚,解鹏,张哲.离体猪肝胆道内射频消融时毗邻胆道热休克蛋白70表达情况[J].转化医学杂志,2018,7(3):150-152
离体猪肝胆道内射频消融时毗邻胆道热休克蛋白70表达情况
The expression of heat shock protein 70 in ex-vivo porcine liver biliary after catheter radiofrequency ablation
  
DOI:
中文关键词:  热休克蛋白70  导管消融术  猪肝  胆管
英文关键词:Heat shock protein 70 (HSP70)  Catheter ablation  Porcine liver  Bile duct
基金项目:北京市科委市民健康培育项目(Z151100003915112);中国人民解放军海军总医院人才引进专项基金(RCYJZX-009)
作者单位
杨超 海军总医院介入医学科 
卢伟 海军总医院介入医学科 
马军朋 海军总医院介入医学科 
杨剑 海军总医院介入医学科 
蒋富强 海军总医院介入医学科 
杜鹏 海军总医院介入医学科 
张嘉诚 海军总医院介入医学科 
解鹏 海军总医院介入医学科 
张哲 海军总医院介入医学科 
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中文摘要:
      目的 探讨采用不同功率胆管内射频消融时,毗邻消融靶区的胆管组织热休克蛋白(heat shock protein,HSP)70表达的差异及意义。方法 15个新鲜离体猪肝随机分为3组(胆囊及胆总管中上段完整保留),将收集的人胆汁注入猪肝胆道系统内,然后将射频消融导管经胆总管引入肝门区胆管内。所有猪肝均消融120 s,第1、2、3组分别采用10 W、12 W、14 W功率消融。消融后取距远端消融电极1cm、2cm处胆管组织,采用免疫组化S-P法检测15例猪肝共30例胆管组织中HSP70的表达。结果 ①距远端消融电极1cm处胆管组织HSP70表达:1级染色4例(26.7%),2级染色6例(40%),3级染色5例(33.3%);距远端消融电极2 cm处胆管组织HSP70表达:1级染色10例(66.7%),2级染色4例(26.7%),3级染色1例(6.6%);②消融区毗邻胆管(距远端消融电极1cm)HSP70表达强度与消融功率呈正相关(r=0.755,P<0.001);距远端消融电极1cm、2cm处胆管组织HSP70表达强度差异有统计学意义(Z=-2.334,P<0.05)。结论 胆管内射频消融可导致消融区毗邻胆管组织HSP70表达,随着消融功率的增加,毗邻消融区胆管组织内HSP70表达强度亦随之增加;远离消融区域胆管(距离远端消融电极≥2cm)HSP70表达无明显增多。
英文摘要:
      Objective To study the expression of heat shock protein 70 (HSP70) in biliary of adjacent ablation area while catheter radiofrequency ablation (RFA) in ex-vivo porcine liver biliary under different power parameters. Methods Fifteen fresh ex-vivo porcine liver bile duct systems (gallbladder and common bile duct preserved) filled with human bile were divided into 3 group randomly. Then, the RFA catheter was inserted into the hilar biliary through the common bile duct. All the ablation time were 120 s, the power of 10 W, 12 W, and 14 W were used for each group respectively. The expression of HSP70 in biliary of 1 cm,2 cm away from the distal pole were examined by immunohistochemical S-P method. Results ①The expression level of HSP70 in biliary of 1 cm away from the distal pole showed 4 cases in the first stage (26.7%), 6 in second stage (40%), and 5 in third stage (33.3%); The expression level of HSP70 in biliary of 2 cm away from the distal pole showed 10 cases in the first stage (66.7%), 4 in second stage (26.7%), and 1 in third stage (6.6%); ②The HSP70 expression level in biliary of 1cm away from the pole showed a positive correlation with the RFA power (r=0.755, P<0.001). Rank sum test showed statistical difference between the HSP70 expression level in biliary of 1cm and 2 cm away from the distal pole (Z=-2.334, P<0.05). Conclusion The HSP70 expression in biliary of adjacent RFA area could be induced by the catheater RFA. More HSP70 expressed with increased power while no more HSP70 expression were found in biliary of 2cm away from the distal pole.
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