孙坚萍,高梦丹,覃岭,代艳超,乔桂芳,张永宏.样本资源库中肝病患者和HIV感染者外周血单个核细胞分离效果比较[J].转化医学杂志,2018,7(4):207-211
样本资源库中肝病患者和HIV感染者外周血单个核细胞分离效果比较
The results of peripheral blood mononuclear cells isolated from liver disease individuals and HIV infected individuals
  
DOI:
中文关键词:  肝病  HIV  感染  外周血单个核细胞
英文关键词:
基金项目:国家传染病重大专项(2017ZX10202102-003-003,2015ZX10004801-001)
作者单位
孙坚萍 首都医科大学附属北京佑安医院生物医学信息中心 
高梦丹 首都医科大学附属北京佑安医院生物医学信息中心 
覃岭 首都医科大学附属北京佑安医院生物医学信息中心 
代艳超 首都医科大学附属北京佑安医院生物医学信息中心 
乔桂芳 首都医科大学附属北京佑安医院生物医学信息中心 
张永宏 首都医科大学附属北京佑安医院生物医学信息中心 
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中文摘要:
      目的样本资源库中不同患者分离的外周血单个核细胞(peripheral blood mononuclear cell,PBMC)保存效果不同,从而影响后续科学研究。对肝病患者和人类免疫缺陷病毒(human immunodeficiency virus,HIV)感染者PBMC分离效果进行分析比较,有助于更好的对患者PBMC细胞质量进行控制。方法121例标本根据疾病诊断分为肝病患者35例,HIV感染者51例,对照组35例。淋巴细胞分离液密度梯度离心法分离PBMC,吖啶橙/碘化丙啶双染色法检测PBMC的活细胞、总细胞和细胞活性。结果3组人群PBMC总细胞数结果显示对照组分离细胞最多(1.54×106, 9.03×105~3.53×106),其次为HIV感染者人群(1.08×106, 3.72×105~2.82×106),最后为肝病患者(8.49×105, 3.61×105~2.30×106),3组之间差异具有统计学意义(P<0.05)。活细胞结果显示对照组分离后活细胞浓度最高(1.53×106, 8.79×105~3.51×106),其次为HIV感染者人群(1.06×106, 3.46×105~2.77×106),最后为肝病患者(8.44×105, 3.54×105~2.25×106),3组之间差异具有统计学意义(P<0.05)。细胞活性结果显示对照组细胞活性(98.40±0.39)高于肝病患者(97.28±0.33)和HIV感染者(96.86±0.37),3组之间差异具有统计学意义(P<0.05)。结论肝病患者和HIV感染者在分离PBMC活细胞、总细胞和细胞活性方面均要低于正常对照组。
英文摘要:
      Objective The quality of isolated peripheral blood mononuclear cells (PBMC) can influence the preservation of PBMC, thus affect the subsequent scientific research. Liver disease individuals and HIV infected individuals are the main study population in hepatitis/AIDS Biobank. Analysis the PBMC of this two groups could help to better control the quality of PBMC. Methods One hundred and twenty-one samples including 35 liver disease individuals, 51 HIV infected individuals and 35 control were analysed. Ficoll density gradient centrifugation to isolate PBMC, AO/PI double staining method to detect the living cells, total cells and cell viability. Results The total number of cells in the control group (1.54×106, 9.03×105—3.53×106) were the highest, followed by HIV infected individuals (1.08×106, 3.72×105—2.82×106), and then the liver disease individuals (8.49×105, 3.61×105—2.30×106), P<0.05. Living cells in the control group were the highest (1.53×106, 8.79×105—3.51×106), followed by HIV infected individuals (1.06×106, 3.46×105—2.77×106), and the liver disease individuals(8.44×105, 3.54×105—2.25×106), P<0.05. The cell viability of the control (98.40±0.39) was higher than that of liver disease individuals (97.28±0.33) and HIV infected individuals (96.86±0.37), P<0.05. Conclusion The PBMC isolated from liver disease individuals and HIV infected individuals were different with control, both the number and the cell viability were lower than those of control.
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